1. Names:

Synonyms:

WWL70;N-Methyl-N-[[3-(4-pyridinyl)phenyl]methyl]-4'-(aminocarbonyl)[1,1'-biphenyl]-4-ylcarbamicacidester

 

  1. WWL70 Usage:

Identified Uses: Laboratory chemicals, Manufacture of substances

 

  1. What is the dosage of WWL70 ?

Solubility & Usage Info:

DMSO to 10 mM, clear (warmed)

Stability and Solubility Advice:

Some solutions can be difficult to obtain and can be encouraged

by rapid stirring, sonication or gentle warming (in a 45-60°C

water bath).

Information concerning product stability, particularly in solution,

has rarely been reported and in most cases we can only offer a

general guide. Our standard recommendations are:

SOLIDS: Provided storage is as stated on the product label and

the vial is kept tightly sealed, the product can be stored for up to

6 months from date of receipt.

SOLUTIONS: We recommend that stock solutions, once

prepared, are stored aliquoted in tightly sealed vials at -20°C or

below and used within 1 month. Wherever possible solutions

should be made up and used on the same day.

 

  1. How WWL70(CAS 947669-91-2) works?

For example, The hydrolytic activity and the levels of 2-AG in BV2 cells were measured by radioactivity assay and liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). The expression of cyclooxygenase-2 (COX-2) and prostaglandin E2 (PGE2) synthases in microglia treated with lipopolysaccharide (LPS) with/without WWL70 was determined by western blot and quantitative RT-PCR. The conversion of 2-AG to PGE2 or PGE2-glyceryl ester (PGE2-G) was assessed by enzyme-linked immunoassay (EIA) or LC-MS/MS. The involvement of ABHD6 in PGE2 production was assessed using pharmacological inhibitors and small interfering RNA (siRNA). The effect of WWL70 on PGE2biosynthesis activity in the microsome fraction from BV2 cells and experimental autoimmune encephalopathy (EAE) mouse brain was also examined.

We found that WWL70 suppressed PGE2 production in LPS-activated microgliavia cannabinoid receptor-independent mechanisms, although intracellular levels of 2-AG were elevated by WWL70 treatment. This reduction was not attributable to WWL70inhibition of ABHD6, given the fact that downregulation of ABHD6 by siRNA or use of KT182, an alternative ABHD6 inhibitor failed to suppress PGE2 production. WWL70 attenuated the expression of COX-2 and PGES-1/2 leading to the downregulation of the biosynthetic pathways of PGE2 and PGE2-G. Moreover, PGE2 production from arachidonic acid was reduced in the microsome fraction, indicating that WWL70also targets PGE2 biosynthetic enzymes, which are likely to contribute to the therapeutic mechanisms of WWL70 in the EAE mouse model.

WWL70 is an anti-inflammatory therapeutic agent capable of inhibiting PGE2 and PGE2-G production, primarily due to its reduction of COX-2 and microsomal PGES-1/2 expression and their PGE2 biosynthesis activity in microglia cells, as well as in the EAE mouse brain.

 

 

5.Warning:

5.1 Please note that tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due toslight batch-to-batch variations.

5.2 If inhaledremove to fresh air and monitor breathing. If breathing becomes difficult, give oxygen. If breathing stops, give artificial respiration. Consult a doctor; In case of skin contact, Immediately wash skin with copious amounts of soap and water for at least 15 minutes. Remove contaminated clothing and shoes and wash before reuse. Consult a doctor; In case of eye contact Flush with copious amounts ofwater for at least 15 minutes. Consult a doctor; If swallowed,rinse mouth with water.Do not induce vomiting unless directed to do so by medical personnel. Never give anything by mouth to an unconscious person. Consult a doctor.

5.3 Most important symptoms and effects, both acute and delayed

To the best of our knowledge, the chemical, physical and toxicological properties have not been thoroughly investigated.

5.4 Indication of immediate medical attention and special treatment needed, call thedoctor immediately.

5.5 Precautions for safe handling,use in a chemical fume hood, with air supplied byan independent system. Avoid inhalation, contact with eyes, skin and clothing. Avoidthe formation of dust and aerosols. Use in a well-ventilated area. Keep away fromsources of ignition. Avoid prolonged or repeated exposure.

5.6 Conditions for safe storage, including any incompatibilities.

Store in cool, well-ventilated area. Keep away from direct sunlight. Keep container tightly sealed until ready for use. Recommended storage temperature: Store at +4°C

5.7 Specific end uses: Use in a laboratory fume hood where possible.

 

6.WWL70:

Min order 10grams.

The inquiry on normal quantity(Within 1kg) can be sent out in 12 hours after payment.

For larger order can be sent out in 3 working days after payment.